糖苷及碳水化合物_武汉科斯坦生物科技有限公司

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产品名称： 邻硝基苯-β-D-吡喃半乳糖苷 ONPG
产品货号： CST19497
货期：现货
价格与订购： 285
数量：

库存： 10
规格： 5g 25g

产品信息
如何订购

产品描述

ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

靶点活性

Others

体外活性

To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1]. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity[1].

别名

2-Nitrophenyl β-D-galactopyranoside , 2-硝基苯-beta-D-半乳糖苷

纯度

99.79%

分子量

301.25

分子式

C12H15NO8

CAS No

369-07-3

外观

White or white-like powder

溶液颜色(1%水溶液)

Colorless to light yellowicon

存储

Powder: -20°C for 3 years In solvent: -80°C for 2 years

溶解度

H2O: 7.4 mg/mL (24.56 mM), Need ultrasonic ( < 1 mg/ml refers to the product slightly soluble or insoluble )

参考文献

1. Zhang X, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin. BMC Microbiol. 2013 Oct 24;13:237.

Note

For research use only .