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- 产品名称: Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
- 产品货号: CST26444
- 货期: 现货
- 价格与订购: 4800
- 数量:
- 规格: 100μg
- 产品信息
- 如何订购
Catalog Number
CST26444
Gene Symbol
NOTCH1, AOS5, AOVD1,TAN1, hN1
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Description
Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
Background
NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
Immunogen
A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
Applications
ELISA, WB, IF, IHC
Recommended Dilutions
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates.
Immunoprecipitation/Western Blot
![](/storage/0000/f91d6489ded1f28cf63f54d3f04c13c7.jpg)
Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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Immunofluorescence:
![](/storage/0000/b697aa9860950353bf357e840ad5abe3.jpg)
Immunofluorescence of cells expressing NOTCH1 proteins with Anti NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP NOTCH1(WT) plasmid (left column) or pCDNA3-GFP NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.