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- 产品名称: Anti SMAD4(D351H) Mouse Monoclonal Antibody
- 产品货号: CST26418
- 货期: 现货
- 价格与订购: 4800
- 数量:
- 规格: 100μg
- 产品信息
- 如何订购
Catalog Number
CST26418
Gene Symbol
SMAD4, DPC4, JIP, MADH4, MYHRS
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Description
Anti SMAD4(D351H) Mouse Monoclonal Antibody
Background
SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-? signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominantdisease juvenile polyposis syndrome (JPS).
Immunogen
A synthetic peptide from the internal region of SMAD4 which includes the mutation of D351H, human origin.
Applications
ELISA, WB, IHC
Recommended Dilutions
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Recognizes D351H mutant, but not wild-type SMAD4 of vertebrates.
Immunoprecipitation/Western Blot
![](/storage/0000/1f661710639633d3ceb0b0e9bed7718d.jpg)
Western blot analysis of recombinant SMAD4(D351H) and wildtype proteins. Purified His-tagged SMAD4(D351H) protein (lane1) and corresponding wild-type protein (1-552aa, 65kd) (lane2) were blotted with anti SMAD4(D351H) monoclonal antibody (Cat. #26418).
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Immunofluorescence:
![](/storage/0000/3b3ad6641868fb1e6c30c9e1e3080d64.jpg)
Immunofluorescence of cells expressing SMAD4 proteins with anti SMAD4(D351H) antibody. HEK293T cells were transfected with pCDNA3-GFP SMAD4(D351H) plasmid , pCDNA3-GFP SMAD4(WT) plasmid or pCDNA3-GFP SMAD4(D351N) plasmid, then fixed and stained with anti SMAD4(D351H) monoclonal antibody (Cat. #26418).
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.