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- 产品名称： Anti Vimentin Mouse Monoclonal Antibody
- 产品货号： 26054
- 货期： 现货
- 价格与订购： 2800
- 数量：
库存： 5
- 规格： 100μg
- 产品信息
- 如何订购
Catalog Number
26054
Gene Symbol
VIM
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Description
Anti Vimentin Mouse Monoclonal Antibody
Background
Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Immunogen
A synthetic peptide from the internal region of human vimentin protein.
Applications
ELISA, WB, IHC, IP
Recommended Dilutions
ELISA: 1:1000-1:5000 ;WB: 1:100-1:1000 ;IHC: 1:50-1:100
Concentration
1 mg/ml
Host
mouse
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Format
Liquid
Clonality
Monoclonal
Purity
Purified from ascites
Preservative
no
Constituents
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity
Anti vimentin antibody recognizes vimentin of vertebrates.
Western blot:
Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti vimentin monoclonal antibody (Cat.# 26054).
Immunohistochemical analysis of paraffin-embedded human renal cancer tissue with anti-vimentin monoclonal antibody (Cat. #26054). Tissue samples were fixed
with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:100 dilution) overnight at 4°C.A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody
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Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles
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Note
For Research Use Only.
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