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- 产品名称： RheB Activation Assay Kit
- 产品货号： 81201
- 货期： 现货
- 价格与订购： 6800
- 数量：
库存： 5
- 规格： 96T
- 产品信息
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Product Description
Small GTPases are a super-family of cellular signaling regulators. RheB is a member of the Ras-superfamily GTPases. RheB has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related. Currently there is no direct assay to measure the activation of RheB GTPases.
Bioyears Biosciences RheB Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes RheB-GTP, but not RheB-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility. These anti RheB GTP monoclonal antibodies can also be used to monitor the activation of RheB in cells and in tissues by immunohistochemistry.
Bioyears Biosciences RheB Activation Assay Kit provides a simple and fast method to monitor the activation of RheB. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences RheB Activation Assay Kit bases on the configuration-specific anti RheB-GTP monoclonal antibody to measure the active RheB-GTP levels, either from cell extracts or from in vitro GTPγS loading RheB activation assays. Briefly, anti active RheB mouse monoclonal antibody will be incubated with cell lysates containing RheB-GTP. The bound active RheB will then be pulled down by protein A/G agarose. The precipitated active RheB will be detected by immunoblot analysis using anti RheB rabbit polyclonal antibody.
Kit Components
1. Anti active RheB, Mouse Monoclonal Antibody (Catalog No. 26910): One vial – 22 µL (1mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody specifically recognizes RheB-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30302): One bottle – 30 mL of 250 mM Tris-HCl, pH 8,
750mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti RheB, Rabbit Polyclonal Antibody (Catalog No. 21098): One vial – 100 µL (1 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30303): One vial –100 µl at 10 mM, use 5 µL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 µl at 100 mM, use 5 µL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4ºC until their expiration dates.
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Note
For Research Use Only.
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