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- 产品名称： Rab5 Activation Assay Kit
- 产品货号： 83701
- 货期： 现货
- 价格与订购： 6800
- 数量：
库存： 5
- 规格： 96T
- 产品信息
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Product Description
Small GTPases are a super-family of cellular signaling regulators. Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Currently there is no direct assay to measure the activation of Rab5 GTPases.
Bioyears Biosciences Rab5 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Rab5 GTP, but not Rab5 GDP, and a Rab5 specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
These anti Rab5 GTP monoclonal antibodies can also be used to monitor the activation of Rab5 in cells and in tissues by immunohistochemistry.
Bioyears Biosciences Rab5 Activation Assay Kit provides a simple and fast method to monitor the activation of Rab5. Each kit provides sufficient quantities to perform 20 assays.
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Assay Principle
Bioyears Biosciences Rab5 Activation Assay Kit bases on the configuration-specific anti Rab5 GTP monoclonal antibody to measure the active Rab5 GTP levels, either from cell extracts or from in vitro GTPγS loading Rab5 activation assays. Briefly, anti-active Rab5 mouse monoclonal antibody will be incubated with cell lysates containing Rab5 GTP. The bound active Rab5 will then be pulled down by protein A/G agarose. The precipitated active Rab5 will be detected by immunoblot analysis using antiRab5 specific rabbit polyclonal antibody.
Kit Components
1. Anti-active Rab5, Mouse Monoclonal Antibody (Catalog No. 26911): One vial – 22 µL (1
mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Rab5-GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 µL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 250 mM Tris-HCl, pH 8, 750mM NaCl, 50 mM MgCl2, 5 mM EDTA, 5% Triton X-100.
4. Anti-Rab5, Rabbit polyclonal Antibody (Catalog No. 21151): One vial – 100 µL (0.2 mg/ml)
in PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30302): One vial –100 μl at 10 mM, use 5 µL of GTPγS for
GTP-labeling of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 µL of GDP for
GDP-labeling of 0.5 mL of cell lysate.
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Storage
Store all kit components at 4ºC until their expiration dates.
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Note
For Research Use Only.
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