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- 产品名称: Anti-Caspase-3 Rabbit Polyclonal Antibody
- 产品货号: CSER30804
- 货期: 现货
- 价格与订购: 1500
- 数量:
- 规格: 50μl 100μl
- 产品信息
- 如何订购
产品描述
Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
产品名称
Anti-Caspase-3 Rabbit Polyclonal Antibody
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分子量
Predicted band size: 32 kDa
种属反应性
Human,Mouse
验证应用
WB,IF-Cell,IHC-P,FC
抗体类型
兔多抗
免疫原
Synthetic peptide within N-terminal human Caspase-3.
偶联
Non-conjugated
形态
Liquid
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浓度
1 mg/mL.
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
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纯化方式
Immunogen affinity purified.
亚细胞定位
Nucleus, cytoplasm,
数据链接
SwissProt: P42574 Human
其它名称
Caspase3;A830040C14Rik antibody;Apopain antibody more
应用
WB: 1:500-1:1,000;IF-Cell: 1:200;IHC-P: 1:200;FC: 1:100-1:200
icon 图谱说明
![](/storage/0000/b4a791b8097a11b93dbec8df2d008ace.jpg)
Fig1: ICC staining of Caspase-3 in HepG2 cells (green).
Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.icon
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背景文献
1. "In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains." Fernandes-Alnemri T., Armstrong R.C., Krebs J.F., Srinivasula S.M., Wang L., Bullrich F., Fritz L.C., Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996)
2. "Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality." Lee D., Long S.A., Adams J.L., Chan G., Vaidya K.S., Francis T.A., Kikly K., Winkler J.D., Sung C.-M., Nuttall M.E.J. Biol. Chem. 275:16007-16014(2000)
icon Note
For research use only .