Gut restricted-7 (GR-7) is a potent, covalent, orally administered, gut-selective bile salt hydrolase (BSH) inhibitor with tissue specificity that localizes to the gut. Gut restricted-7 reduces BSH activity in gut bacteria and decreases levels of unconjugated bile acids in mouse feces, making it suitable for research into metabolism, inflammation, and intestinal diseases.

方法：6 种人肠道共生菌（3 种革兰阴性：B. theta， B. fragilis， B. vulgatus；3 种革兰阳性：L. plantarum， C. perfringens， B. adolescentis），将对数生长期前的细菌与 Gut restricted-7（100 μM）和混合胆汁酸底物（100 μM）同时孵育，监测 21 小时 内的去结合反应。 结果：在所有 6 种菌株中，Gut restricted-7 几乎完全抑制了胆汁酸去结合活性。