CSD00084

Infliximab

>95%

1mg/ml

PBS buffer PH7.5

CHO cells

Please contact with the lab for this information.

Infliximab is a humanized antibody against tumor necrosis factor α (TNF-α) that is used in the treatment of Crohn's disease and rheumatoid arthritis.Infliximab is a IgG1κ monoclonal antibody that binds to soluble and transmembrane forms of TNF-α with high affinity to disrupt the pro-inflammatory cascade signalling.Binding of the antibody to TNF-α prevents TNF-α from interacting with its receptors.Infliximab does not neutralize TNF-β (lymphotoxin-α),a related cytokine that utilizes the same receptors as TNF-α Label.Blocked actions of TNF-α further leads to downregulation of local and systemic pro-inflammatory cytokines (i.e. IL-1, IL-6),reduction of lymphocyte and leukocyte migration to sites of inflammation, induction of apoptosis of TNF-producing cells (i.e. activated monocytes and T lymphocytes),increased levels of nuclear factor-κB inhibitor, and reduction of reduction of endothelial adhesion molecules and acute phase proteins 3. Its inhibitory actions on TNF-α was demonstrated in human fibroblasts,endothelial cells, neutrophils, B and Tlymphocytes and epithelial cells Label.Infliximab also atteunates the production of tissue degrading enzymes synthesized by synoviocytes and/or chondrocytes. According to a transgenic mice study that developed polyarthritis due to consitutive levels of human TNF-α,infliximab decreased synovitis and joint erosions in collagen-induced arthritis and allows eroded joints to heal Label.

TA-650,cA2

TNF-alpha[Homo sapiens]

To some of the polyclonally or antigen-specific-stimulated cell cultures, 1, 10 or 100 lg/ml of infiximab (MSD, Whitehouse Station, NJ) was added at the time for T cell stimulation or 48 h after T cell stimulation. Consecutive cell culture supernatants were collected and stored every 24 h or at the end of the cell culture. The T cell proliferation was measured at the end of cell culture by pulsing the cells with 0.5 lCi of [3H] thymidine/well (Amersham, Arlington Heights) for the last 8 h, and the incorporated radioactivity was analysed using a scintillation counter.

Chimeric

IgG1-kappa

170277-31-3

For research use only .