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- 产品名称： Anti-DNA-RNA Hybrid [S9.6] Antibody
- 产品货号： CS2112
- 货期： 现货
- 价格与订购： 2800
- 数量：
库存： 100
- 规格： 100μl
- 产品信息
- 如何订购
Synonyms
DNA-RNA hybrid; RNA/DNA hybrid
Attribute
Mouse Monoclonal Antibody
Isotype
Mouse IgG2a
Purity
Protein A/G Purification
Application
ELISA, IP, IF, CHIP, Dot Blot
Immunogen
ΦX174 bacteriophage-derived synthetic DNA/RNA
Buffer
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Storage
Store at -20℃. Do not aliquot
Recommended Dilution
IP: 1:50-500IF: 1: 200
Background
The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity.
Immunofluorescentanalysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500 and Alexa Fluor 647- conjugatedAffiniPure Goat Anti-Mouse IgG(H+L)
Chromatin immunoprecipitationanalysis of HeLa cells
genomic DNA(gDNA)using DNA-RNA hybrid Mouse Monoclonal
Antibody [S9.6] at dilution of 1:200
Note
For research use only .
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Amily
