DYKDDDDK 

Rabbit Polyclonal Antibody 

 

Antigen Affinity Purification 

ELISA, WB, IP, IF

34kDa 

34kDa

Human,Mouse,Rat,Zebra fish 

Peptide DYKDDDDK

 PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 

Store at -20℃. Do not aliquot 

WB: 1:2000-50000IP: 3ug/sampleIF: 1:100-200 

FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.

                                                                      

Hela cells transfected withthe plasmid over- expressing                               Western blotof Flag-tagged fusion protein with 

3×FLAG Fusion Protein, using anti Flag antibody at1:200 dilution       anti-Flag antibody（Cat#：CS3064） at dilution of 1:1000

For research use only .