一、重组抗体表达

重组抗体被广泛地应用在科学研究、免疫诊断及治疗性抗体领域。科斯坦生物生物推出CASRATM保证型重 组抗体表达服务。科斯坦生物生物自有的哺乳表达系统高产量pATX系列载体及驯化293系列和CHO系列细胞， 能实现全长抗体以及多种形式的重组抗体片段的高效分泌表达。无需免疫，可实现单克隆抗体及其它 形式重组抗体的高产和可重复生产。

二、服务内容

CSRA^TM保证型重组抗体表达服务内容

三、服务流程

四、武汉科斯坦生物优势

1.科斯坦生物拥有抗体药物研发专家和技术支持团队，可对您感兴趣的抗体讲行个性化的序列优化设计，提高重组抗体表达量。全长抗体分泌表达量可达100-300mg/L实现一次表达终身受益。

2.丰富的抗体表达经验和技术团队，已经成功表达过多种形式的全长抗体(HumanIgG1~4，Mous e IgG2b,MouseIgG2a,RabbitIgG等)抗体片段(scfv，Fab,tandem-scfv，VHH，Fc融合蛋白。 bi-specificantibody等)。

3.科斯坦生物拥有多种服务平台，在实现重组抗体设计表达和纯化的同时，同时可以实现抗体的活性验证。目前已经设计完成了多个嵌合抗体的表达及活性验证，并对部分人鼠嵌合抗体进行人源化设计验证。真正实现一站式服务。

五、案例展示

Recombinant Antibody Production Cases

Expression caseI:scFv

Expression system:Bsubtilis ExpressionSystem

Service Type:Recombinant Protein Production

Conclusion: Total obtained 37mg scFy from 1L scale up purification, purity is about 90%.

Figure 1. Expression result

Figure 2. Target scFv purification profile

Figure 3. Final sample QC

Expression caseⅡ:Bi-specific Antibody

Expression System:Mammalian 293F Expression System

Service Type: Recombinant Antibody Production

Conclusion:Bi-specific antibody A total obtained 17.35mg per liter of culture, purity> 90% Bi-specific antibody B total obtained 16.23mg per liter of culture, purity> 85%.

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression casellI: IgG-like bi-specific antibody

Expression System:Mammalian CHO-S Expression System

Service Type:Recombinant Antibody Production

Conclusion:Total obtained IgG-like: 104mg from 1L scale up purification, purity> 90%

Figure 1. Expression result

Figure 2. Target protein purification profile

Figure 3. Final sample QC

Expression caselII: HH expressed with tag and tag removal

Service Type:Recombinant Antibody Production

Conclusion: Total obtained VHH without any tag: 13.2mg from 1L scale up purification after tag removal, purity> 90%

Target protein purification profile in native condition.

Tag cleavage results.

1. Before cleavage sample 2. After cleavage sample

Final sample QC

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